Dataset Information

Validation of an automated assay for measurement of bovine plasma ceruloplasmin.

ABSTRACT: Ceruloplasmin (Cp) plays an important role in copper transport and iron metabolism, as well as Cp is also an indicator for the health status of dairy cows. The present study reports the validation of an automated assay to assess the plasma Cp in dairy cows. Plasma Cp levels were determined in 40 Holstein cows and intra- and inter-assay precision, accuracy, detection limit, and clinical validation of the assay were determined. Intra- and inter-assay coefficients of variation were < 2% and < 7%, respectively. The results were linear when serial sample dilutions were tested (r = 0.999, P < 0.001). The detection limit was lower than what could be measured in plasma from healthy cows. Increased plasma Cp levels were found in cows with inflammatory diseases. The method validated in this study is precise, simple, and fast and can be easily adapted to biochemical automated analysers. Furthermore, the promising results obtained with this protein will contribute to a wider use of Cp determination in bovine practice.

SUBMITTER: Hussein HA

PROVIDER: S-EPMC6647283 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Validation of an automated assay for measurement of bovine plasma ceruloplasmin.

Hussein Hussein Awad HA Bäumer Jacqueline J Staufenbiel Rudolf R

Acta veterinaria Scandinavica 20190722 1

Ceruloplasmin (Cp) plays an important role in copper transport and iron metabolism, as well as Cp is also an indicator for the health status of dairy cows. The present study reports the validation of an automated assay to assess the plasma Cp in dairy cows. Plasma Cp levels were determined in 40 Holstein cows and intra- and inter-assay precision, accuracy, detection limit, and clinical validation of the assay were determined. Intra- and inter-assay coefficients of variation were < 2% and < 7%, r ...[more]

PMID: 31331378

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Project description:BackgroundThe recent promise of disease-modifying therapies for Alzheimer's disease (AD) has reinforced the need for accurate biomarkers for early disease detection, diagnosis and treatment monitoring. Advances in the development of novel blood-based biomarkers for AD have revealed that plasma levels of tau phosphorylated at various residues are specific and sensitive to AD dementia. However, the currently available tests have shortcomings in access, throughput, and scalability that limit widespread implementation.MethodsWe evaluated the diagnostic and prognostic performance of a high-throughput and fully-automated Lumipulse plasma p-tau181 assay for the detection of AD. Plasma from older clinically unimpaired individuals (CU, n = 463) and patients with mild cognitive impairment (MCI, n = 107) or AD dementia (n = 78) were obtained from the longitudinal Stanford University Alzheimer's Disease Research Center (ADRC) and the Stanford Aging and Memory Study (SAMS) cohorts. We evaluated the discriminative accuracy of plasma p-tau181 for clinical AD diagnosis, association with amyloid β peptides and p-tau181 concentrations in CSF, association with amyloid positron emission tomography (PET), and ability to predict longitudinal cognitive and functional change.ResultsThe assay showed robust performance in differentiating AD from control participants (AUC 0.959, CI: 0.912 to 0.990), and was strongly associated with CSF p-tau181, CSF Aβ42/Aβ40 ratio, and amyloid-PET global SUVRs. Associations between plasma p-tau181 with CSF biomarkers were significant when examined separately in Aβ+ and Aβ- groups. Plasma p-tau181 significantly increased over time in CU and AD diagnostic groups. After controlling for clinical diagnosis, age, sex, and education, baseline plasma p-tau181 predicted change in MoCA overall and change in CDR Sum of Boxes in the AD group over follow-up of up to 5 years.ConclusionsThis fully-automated and available blood-based biomarker assay therefore may be useful for early detection, diagnosis, prognosis, and treatment monitoring of AD.

Dataset Information

Validation of an automated assay for measurement of bovine plasma ceruloplasmin.

Publications

Validation of an automated assay for measurement of bovine plasma ceruloplasmin.

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