Crosstalk between chromatin structure, cohesin activity and transcription.

ABSTRACT:

Background

A complex interplay between chromatin and topological machineries is critical for genome architecture and function. However, little is known about these reciprocal interactions, even for cohesin, despite its multiple roles in DNA metabolism.

Results

We have used genome-wide analyses to address how cohesins and chromatin structure impact each other in yeast. Cohesin inactivation in scc1-73 mutants during the S and G2 phases causes specific changes in chromatin structure that preferentially take place at promoters; these changes include a significant increase in the occupancy of the - 1 and + 1 nucleosomes. In addition, cohesins play a major role in transcription regulation that is associated with specific promoter chromatin architecture. In scc1-73 cells, downregul

SUBMITTER: Maya-Miles D 

PROVIDER: S-EPMC6647288 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature