Visualization and quantification of mitochondrial structure in the endothelium of intact arteries.

ABSTRACT:

Aim

To quantify the mitochondrial structure of ECs in intact arteries vs. cultured cells.

Methods and results

Cre-stop mito-Dendra2 mice, expressing the fluorescent protein Dendra2 in the mitochondrial matrix only, were used to label EC mitochondria using Cre-recombinase under the control of the VE-cadherin promoter. Conduit arteries, resistance arterioles and veins were fixed, mounted on glass slides and fluorescent images were obtained using a laser scanning confocal microscope (ex 488 nm; em 550 nm). ImageJ was used to calculate form factor (FF) and aspect ratio (AR) of the mitochondrial segments. Mitochondrial fragmentation count (MFC) was calculated by counting non-contiguous mitochondrial particles and dividing by the number of pixels which comprise the mitochondrial n

SUBMITTER: Durand MJ 

PROVIDER: S-EPMC6648341 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature