Selective Amino Acid-Only in Vivo NMR: A Powerful Tool To Follow Stress Processes.

ABSTRACT: In vivo NMR of small ¹³C-enriched aquatic organisms is developing as a powerful tool to detect and explain toxic stress at the biochemical level. Amino acids are a very important category of metabolites for stress detection as they are involved in the vast majority of stress response pathways. As such, they are a useful proxy for stress detection in general, which could then be a trigger for more in-depth analysis of the metabolome. ¹H-¹³C heteronuclear single quantum coherence (HSQC) is commonly used to provide additional spectral dispersion in vivo and permit metabolite assignment. While some amino acids can be assigned from HSQC, spectral overlap makes monitoring them in vivo challenging. Here, an experiment typically used to study protein structures is

SUBMITTER: Lane D 

PROVIDER: S-EPMC6648361 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature