In Silico Structural Modeling and Analysis of Elongation Factor-1 Alpha and Elongation Factor-like Protein.

ABSTRACT: Translation elongation factor-1alpha (EF-1α) or its paralog elongation factor-like proteins (EFL) interact with an aminoacyl-transfer RNA (aa-tRNA) to play its essential role in elongation of peptide-chain during protein synthesis. Species usually have either an EF-1α or EFL protein; however, some species have both EF-1α and EFL (dual-EF-containing species). In the dual-EF-containing species, EF-1α appeared to be highly divergent in the sequence. Homology modeling and surface analysis of EF-1α and EFL were performed to examine the hypothesis that the divergent EF-1α in the dual-EF-containing eukaryotes does not strongly interact with aa-tRNA compared to the canonical EF-1α and EFL. The subsequent molecular dynamics simulations were carried out to confirm the validity of modeled structures

SUBMITTER: Sakamoto K 

PROVIDER: S-EPMC6648415 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature