Ratiometric Sensing for Alkaline Phosphatase Based on Two Independent Signals from in Situ Formed Nanohybrids of Semiconducting Polymer Nanoparticles and MnO₂ Nanosheets.

ABSTRACT: Ratiometric sensing systems transduced through independent analyte-sensitive response signals, which are simultaneously obtained from a single material, are highly desired to improve sensing reliability and sensitivity. In this study, a dual-model ratiometric sensing system with fluorescence and second-order light scattering (SOS) as transducing signals has been designed for the ratiometric detection of alkaline phosphatase (ALP). Semiconducting polymer nanoparticles (SPNs) made of poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1',3}-thiadiazole)] are prepared and used as reducing and stabilizing agents to prepare MnO₂ nanosheets in situ through the reduction of KMnO₄. The formed SPNs@MnO₂ nanohybrids exhibit independent fluorescence and SOS r

SUBMITTER: He J 

PROVIDER: S-EPMC6648444 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature