Linear Extrapolation of the Analyte-Specific Light Scattering and Fluorescence Depolarization in Turbid Samples.

ABSTRACT: Anisotropy and depolarization are two interconvertible parameters in fluorescence and light scattering spectroscopy that describe the polarization distribution of emitted and scattered photons generated with linearly polarized excitation light. Whereas anisotropy is more frequently used in fluorescence literature for studying association/dissociation of fluorophore-bearing reagents, depolarization is more popular in the light-scattering literature for investigating the effect of scatterers' geometries and chemical compositions. Presented herein is a combined computational and experimental study of the scattering and fluorescence depolarization enhancement induced by light scattering in turbid samples. The most important finding is that sample light scattering and fluorescence depolarizatio

SUBMITTER: Xu JX 

PROVIDER: S-EPMC6648588 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature