Vibrational Sum-Frequency Scattering as a Sensitive Approach to Detect Structural Changes in Collagen Fibers Treated with Surfactants.

ABSTRACT: Optimizing protocols so that the structure of the collagen fibers in the extracellular matrix remains intact during the decellularization process requires techniques with high structural sensitivity, especially for the surface region of the collagen fibers. Here, we demonstrate that vibrational sum-frequency scattering (SFS) spectroscopy in the protein-specific amide I region provides vibrational spectra and scattering patterns characteristic of protein fiber networks self-assembled in vitro from collagen type I, which are kept in aqueous environments during the analysis. At scattering angles away from the phase-matched direction, the relative strengths of various polarization combinations are highly reproducible, and changes in their ratios can be followed in real time during exposure to

SUBMITTER: Johansson PK 

PROVIDER: S-EPMC6648693 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature