Effects of antiepileptic drugs in a new TSC/mTOR-dependent epilepsy mouse model.

ABSTRACT:

Objective

An epilepsy mouse model for Tuberous Sclerosis Complex (TSC) was developed and validated to investigate the mechanisms underlying epileptogenesis. Furthermore, the possible antiepileptogenic properties of commonly used antiepileptic drugs (AEDs) and new compounds were assessed.

Methods

Tsc1 deletion was induced in CAMK2A-expressing neurons of adult mice. The antiepileptogenic properties of commonly used AEDs and inhibitors of the mTOR pathways were assessed by EEG recordings and by molecular read outs.

Results

Mice developed epilepsy in a narrow time window (10 ± 2 days) upon Tsc1 gene deletion. Seizure frequency but not duration increased over time. Seizures were lethal within 18 days, were unpredictable, and did not correlate to seizure onset, length or fr

SUBMITTER: Koene LMC 

PROVIDER: S-EPMC6649373 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature