Identification of serum microRNAs as potential biomarkers in Pompe disease.

ABSTRACT:

Objective

To analyze the microRNA profile in serum of patients with Adult Onset Pompe disease (AOPD).

Methods

We analyzed the expression of 185 microRNAs in serum of 15 AOPD patients and five controls using microRNA PCR Panels. The expression levels of microRNAs that were deregulated were further studied in 35 AOPD patients and 10 controls using Real-Time PCR. Additionally, the skeletal muscle expression of microRNAs which showed significant increase levels in serum samples was also studied. Correlations between microRNA serum levels and muscle function test, spirometry, and quantitative muscle MRI were performed (these data correspond to the study NCT01914536 at ClinicalTrials.gov).

Results

We identified 14 microRNAs that showed different expression levels in serum s

SUBMITTER: Carrasco-Rozas A 

PROVIDER: S-EPMC6649638 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature