Study of conformational transitions of i-motif DNA using time-resolved fluorescence and multivariate analysis methods.

ABSTRACT: Recently, the presence of i-motif structures at C-rich sequences in human cells and their regulatory functions have been demonstrated. Despite numerous steady-state studies on i-motif at neutral and slightly acidic pH, the number and nature of conformation of this biological structure are still controversial. In this work, the fluorescence lifetime of labelled molecular beacon i-motif-forming DNA sequences at different pH values is studied. The influence of the nature of bases at the lateral loops and the presence of a Watson-Crick-stabilized hairpin are studied by means of time-correlated single-photon counting technique. This allows characterizing the existence of several conformers for which the fluorophore has lifetimes ranging from picosecond to nanosecond. The information on the exis

SUBMITTER: Benabou S 

PROVIDER: S-EPMC6649798 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature