IL23R gene variants in relation to IL17A levels and clinical phenotype in patients with ankylosing spondylitis.

ABSTRACT:

Background

IL23 receptor (IL23R) binding by IL23 is required for the maturation of CD4⁺ cells into Th17 cells and subsequent generation of IL17A and TNF. As IL23R variations contribute to AS susceptibility, we investigated the effect of IL23R variants on cytokine levels and disease measures in an AS cohort.

Methods

This was a cross-sectional study of AS patients (n = 334, 90% B27⁺, age 45 years). IL23R genotyping for three non-synonymous single-nucleotide polymorphisms (rs11209026, protective allele A; rs10489629, protective allele A; and rs11209032, risk allele A) was done by Taqman RT-PCR. IL23, IL17A, TNF and IL6 concentrations were determined by sandwich ELISA. Genotypic associations were analysed with non-parametric methods.

SUBMITTER: Nossent JC 

PROVIDER: S-EPMC6649922 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature