A comparison of Lyse-It to other cellular sample preparation, bacterial lysing, and DNA fragmentation technologies.

ABSTRACT: The ability for safe and rapid pathogenic sample transportation and subsequent detection is an increasing challenge throughout the world. Herein, we describe and use bead-beating, vortex, sonication, 903 protein saver cards, and Lyse-It methods, aiming to inactivate Gram-positive and -negative bacteria with subsequent genome DNA (quantitative Polymerase Chain Reaction) qPCR detection. The basic concepts behind the four chosen technologies is their versatility, cost, and ease of use in developed and underdeveloped countries. The four methods target the testing of bacterial resilience, cellular extraction from general and complex media and subsequent DNA extraction for qPCR detection and amplification. These results demonstrate that conventional high temperature heating, 903 protein saver ca

SUBMITTER: Santaus TM 

PROVIDER: S-EPMC6650070 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature