Long Term Potentiation in Mouse Hippocampal Slices in an Undergraduate Laboratory Course.

ABSTRACT: Long-term potentiation (LTP) is thought to be a critical mechanism underlying learning and memory. Although LTP is now widely performed in neuroscience research laboratories and the theory behind it is taught in many undergraduate courses, it is rare for undergraduate students to have the opportunity to perform LTP experiments themselves. Here, we describe a series of two laboratory sessions in which upper level students learn how to perform LTP experiments in acute hippocampal slices from wild type mice. In Laboratory 1, students practice the techniques necessary to set up the experiments. These techniques include making solutions, pulling glass recording electrodes, performing brain removal, preparing hippocampal slices, and positioning electrodes in area CA1. For Laboratory 2, hippocamp

SUBMITTER: Quinan V 

PROVIDER: S-EPMC6650259 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature