Dynamic Mitochondrial Migratory Features Associated with Calcium Responses during T Cell Antigen Recognition.

ABSTRACT: A T cell clone is able to distinguish Ags in the form of peptide-MHC complexes with high specificity and sensitivity; however, how subtle differences in peptide-MHC structures translate to distinct T cell effector functions remains unknown. We hypothesized that mitochondrial positioning and associated calcium responses play an important role in T cell Ag recognition. We engineered a microfluidic system to precisely manipulate and synchronize a large number of cell-cell pairing events, which provided simultaneous real-time signaling imaging and organelle tracking with temporal precision. In addition, we developed image-derived metrics to quantify calcium response and mitochondria movement. Using myelin proteolipid altered peptide ligands and a hybridoma T cell line derived from a mouse mode

SUBMITTER: He L 

PROVIDER: S-EPMC6650333 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature