Evaluation of cell viability and metabolic activity of a 3D cultured human epidermal model using a dynamic autoradiographic technique with a PET radiopharmaceutical.

ABSTRACT: Quality control of tissues and organs for transplant is important to confirm their safety and effectiveness for regenerative medicine. However, quality evaluation is only carried out using a limited range of inspection criteria, because many of the available evaluation tests are invasive. In order to explore the potential of 2-[¹⁸F]fluoro-2-deoxy-D-glucose ([¹⁸F]FDG)-bioradiography as a non-invasive test for estimation of the safety, soundness, and effectiveness of tissues for transplantation, [¹⁸F]FDG uptake and cell viability or metabolism were investigated using a reconstructed human epidermal model (RHEM). We developed an imaging system, and suitable bioradiographic image acquisition conditions and its effectiveness were investigated. [¹⁸F]FD

SUBMITTER: Sasaki T 

PROVIDER: S-EPMC6650402 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature