Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions.

ABSTRACT: Dysfunction or death of retinal pigment epithelial (RPE) cells is involved in some forms of Retinitis Pigmentosa and in age-related macular degeneration (AMD). Since there is no cure for most patients affected by these diseases, the transplantation of RPE cells derived from human pluripotent stem cells (hPSCs) represents an attractive therapeutic alternative. First attempts to transplant hPSC-RPE cells in AMD and Stargardt patients demonstrated the safety and suggested the potential efficacy of this strategy. However, it also highlighted the need to upscale the production of the cells to be grafted in order to treat the millions of potential patients. Automated cell culture systems are necessary to change the scale of cell production. In the present study, we developed a protocol amenable

SUBMITTER: Regent F 

PROVIDER: S-EPMC6650487 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature