DMSO cryopreservation is the method of choice to preserve cells for droplet-based single-cell RNA sequencing.

ABSTRACT: Combining single-cell RNA sequencing (scRNA-seq) with upstream cell preservation procedures such as cryopreservation or methanol fixation has recently become more common. By separating cell handling and preparation, from downstream library generation, scRNA-seq workflows are more flexible and manageable. However, the inherent transcriptomic changes associated with cell preservation and how they may bias further downstream analysis remain unknown. Here, we present a side-by-side droplet-based scRNA-seq analysis, comparing the gold standard - fresh cells - to three different cell preservation workflows: dimethyl sulfoxide based cryopreservation, methanol fixation and CellCover reagent. Cryopreservation proved to be the most robust protocol, maximizing both cell integrity and low background a

SUBMITTER: Wohnhaas CT 

PROVIDER: S-EPMC6650608 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature