Slowly Reducible Genetically Encoded Green Fluorescent Indicator for In Vivo and Ex Vivo Visualization of Hydrogen Peroxide.

ABSTRACT: Hydrogen peroxide (H₂O₂) plays an important role in modulating cell signaling and homeostasis in live organisms. The HyPer family of genetically encoded indicators allows the visualization of H₂O₂ dynamics in live cells within a limited field of view. The visualization of H₂O₂ within a whole organism with a single cell resolution would benefit from a slowly reducible fluorescent indicator that integrates the H₂O₂ concentration over desired time scales. This would enable post hoc optical readouts in chemically fixed samples. Herein, we report the development and characterization of NeonOxIrr, a genetically encoded green fluorescent indicator, which rapidly increases fluorescence brightness upon reaction

SUBMITTER: Subach OM 

PROVIDER: S-EPMC6650888 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature