Project description:The purpose of this study is to investigate the role of SIRT1 in high-fat diet-induced liver steatosis and insulin resistance. SIRT1 is a nuclear enzyme that could remove an acetyl-group from target proteins by using NAD as co-substrate. Homologs of this protein in yeast and the roundworm C. elegans are able to delay the aging process in response to nutrients. However, the molecular mechanism by which SIRT1 sense the environment to mediate this response are poorly understood. We have shown that when chronically fed with a 40%-fat diet, SIRT1 heterozygous animals gain significantly more weight compared to wild type littermates. They are also hyperinsulimia, more insulin-resistant, and accumulate more lipids in liver. Interestingly, these animals also show signs of premature aging, such as an early appearance of gray fur, defective motor activity, and decreased fertility. In this microarray study, we analyzed the gene expression profiles in the liver of WT low-fat diet, Het low-fat diet, WT high-fat diet, and Het high-fat diet using Agilent Whole Genome Mouse 4x44 multiplex format oligo arrays following the Agilent-1-color microarray-based gene expression analysis protocol. This microarray analysis concluded that SIRT1 Het mice reponsed to the high-fat diet differently from the WT control mice. Liver total RNAs from SIRT1 WT and Het mice that were fed with either a low-fat diet or a high-fat diet for 34 weeks were used for a microarray gene expression study. Three biological replicates for each group were used.

2012-07-31 | E-GEOD-39778 | biostudies-arrayexpress

Editorial: The role of vitamin D in metabolic and cardiovascular health.

Project description: Not available

| S-EPMC10165735 | biostudies-literature

Editorial: The Role of Obesity and Metabolic Syndrome in Couple Infertility.

Project description: Not available

| S-EPMC8631512 | biostudies-literature

Editorial: Impact of hyperglycemia induced oxidative stress in genetics and epigenetics of metabolic diseases.

Project description: Not available

| S-EPMC9909956 | biostudies-literature

Role of FoxO transcription factors in aging and age-related metabolic and neurodegenerative diseases.

Project description:Aging happens to all of us as we live. Thanks to the improved living standard and discovery of life-saving medicines, our life expectancy has increased substantially across the world in the past century. However, the rise in lifespan leads to unprecedented increases in both the number and the percentage of individuals 65 years and older, accompanied by the increased incidences of age-related diseases such as type 2 diabetes mellitus and Alzheimer's disease. FoxO transcription factors are evolutionarily conserved molecules that play critical roles in diverse biological processes, in particular aging and metabolism. Their dysfunction is often found in the pathogenesis of many age-related diseases. Here, we summarize the signaling pathways and cellular functions of FoxO proteins. We also review the complex role of FoxO in aging and age-related diseases, with focus on type 2 diabetes and Alzheimer's disease and discuss the possibility of FoxO as a molecular link between aging and disease risks.

| S-EPMC8561869 | biostudies-literature

Editorial: Epigenetic Regulation in Cardiovascular Diseases.

Project description: Not available

| S-EPMC8787130 | biostudies-literature

Editorial: Casein kinases in human diseases.

Project description: Not available

| S-EPMC9755584 | biostudies-literature

Editorial: Molecular mechanisms of neuropsychiatric diseases.

Project description: Not available

| S-EPMC9773978 | biostudies-literature