Lineage-Specific Evolved MicroRNAs Regulating NB-LRR Defense Genes in Triticeae.

ABSTRACT: Disease resistance genes encoding proteins with nucleotide binding sites and Leucine-Rich Repeat (NB-LRR) domains include many members involved in the effector-triggered immunity pathway in plants. The transcript levels of these defense genes are negatively regulated by diverse microRNAs (miRNAs) in angiosperms and gymnosperms. In wheat, using small RNA expression datasets and degradome datasets, we identified five miRNA families targeting NB-LRR defense genes in monocots, some of which arose in the Triticeae species era. These miRNAs regulate different types of NB-LRR genes, most of them with coil-coiled domains, and trigger the generation of secondary small interfering RNAs (siRNA) as a phased pattern in the target site regions. In addition to acting in response to b

SUBMITTER: Zhang R 

PROVIDER: S-EPMC6651130 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature