Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology.

ABSTRACT:

Background

Plant protoplasts are basic plant cells units in which the pecto-cellulosic cell wall has been removed, but the plasma membrane is intact. One of the main features of plant cells is their strong plasticity, and their propensity to regenerate an organism from a single cell. Methods and differentiation protocols used in plant physiology and biology usually involve macroscopic vessels and containers that make difficult, for example, to follow the fate of the same protoplast all along its full development cycle, but also to perform continuous studies of the influence of various gradients in this context. These limits have hampered the precise study of regeneration processes.

Results

Herein, we present the design of a comprehensive, physiologically relevant, easy-to-us

SUBMITTER: Sakai K 

PROVIDER: S-EPMC6651895 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature