Transcription Factor IRF4 Dysfunction Affects the Immunosuppressive Function of Treg Cells in Patients with Primary Immune Thrombocytopenia.

ABSTRACT:

Background

Th17/Treg balance skews towards Th17 in ITP patient. IRF4 has been highlighted for its close relationship to the immunosuppressive function of Treg cells and the IL-17 synthesis in CD4⁺ T cells. This study was aimed at examining the effects of IRF4 to the Th17/Treg cells in patients with ITP.

Methods

Treg and Teff cells were isolated from PBMCs of newly diagnosed ITP patients. The percentages of CD4⁺CD25^hiFoxp3⁺Treg cells and the CD3⁺CD4⁺IL-17⁺Th17 cells were detected by flow cytometry. After being cultured, the supernatants of Tregs were collected for IL-10 concentration test. The IRF4 levels of Tregs were measured. Teffs were cultured alone or with Tregs for 24 hours. Then the supernata

SUBMITTER: Tang M 

PROVIDER: S-EPMC6652070 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature