WDFY4 is required for cross-presentation in response to viral and tumor antigens.

ABSTRACT: During the process of cross-presentation, viral or tumor-derived antigens are presented to CD8⁺ T cells by Batf3-dependent CD8α⁺/XCR1⁺ classical dendritic cells (cDC1s). We designed a functional CRISPR screen for previously unknown regulators of cross-presentation, and identified the BEACH domain-containing protein WDFY4 as essential for cross-presentation of cell-associated antigens by cDC1s in mice. However, WDFY4 was not required for major histocompatibility complex class II presentation, nor for cross-presentation by monocyte-derived dendritic cells. In contrast to Batf3 ^-/- mice, Wdfy4 ^-/- mice displayed normal lymphoid and nonlymphoid cDC1 populations that produce interleukin-12 and protect against Toxopla

SUBMITTER: Theisen DJ 

PROVIDER: S-EPMC6655551 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature