Rapid metagenomics analysis of EMS vehicles for monitoring pathogen load using nanopore DNA sequencing.

ABSTRACT: Pathogen monitoring, detection and removal are essential to public health and outbreak management. Systems are in place for monitoring the microbial load of hospitals and public health facilities with strategies to mitigate pathogen spread. However, no such strategies are in place for ambulances, which are tasked with transporting at-risk individuals in immunocompromised states. As standard culturing techniques require a laboratory setting, and are time consuming and labour intensive, our approach was designed to be portable, inexpensive and easy to use based on the MinION third-generation sequencing platform from Oxford Nanopore Technologies. We developed a transferable sampling-to-analysis pipeline to characterize the microbial community in emergency medical service vehicles. Our approac

SUBMITTER: Sheahan T 

PROVIDER: S-EPMC6655686 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature