Development of an in vitro media perfusion model of Leishmania major macrophage infection.

ABSTRACT:

Background

In vitro assays are widely used in studies on pathogen infectivity, immune responses, drug and vaccine discovery. However, most in vitro assays display significant differences to the in vivo situation and limited predictive properties. We applied medium perfusion methods to mimic interstitial fluid flow to establish a novel infection model of Leishmania parasites.

Methods

Leishmania major infection of mouse peritoneal macrophages was studied within the Quasi Vivo QV900 macro-perfusion system. Under a constant flow of culture media at a rate of 360μl/min, L. major infected macrophages were cultured either at the base of a perfusion chamber or raised on 9mm high inserts. Mathematical and computational modelling was conducted to estimate medium flow speed, shear stre

SUBMITTER: O'Keeffe A 

PROVIDER: S-EPMC6656416 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature