Comprehensive Immune Monitoring of Clinical Trials to Advance Human Immunotherapy.

ABSTRACT: The success of immunotherapy has led to a myriad of clinical trials accompanied by efforts to gain mechanistic insight and identify predictive signatures for personalization. However, many immune monitoring technologies face investigator bias, missing unanticipated cellular responses in limited clinical material. We present here a mass cytometry (CyTOF) workflow for standardized, systems-level biomarker discovery in immunotherapy trials. To broadly enumerate immune cell identity and activity, we established and extensively assessed a reference panel of 33 antibodies to cover major cell subsets, simultaneously quantifying activation and immune checkpoint molecules in a single assay. This assay enumerates ≥98% of peripheral immune cells with ≥4 positively identifying antigens. Robustness and

SUBMITTER: Hartmann FJ 

PROVIDER: S-EPMC6656694 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature