Real-time PCR and targeted next-generation sequencing in the detection of low level EGFR mutations: Instructive case analyses.

ABSTRACT:

Background

Allele specific real-time PCR and next-generation sequencing (NGS) are widely used to detect somatic mutation in non-small cell lung cancer (NSCLC). Both methods commonly use formalin-fixed paraffin-embedded (FFPE) tissues as diagnostic materials. Real-time PCR has the advantage of being easy to use and more tolerant of variable DNA quality, but has limited multiplex capability. NGS, in contrast, allows simultaneous analysis of many genomic loci while revealing the exact sequence changes; it is, however, more technically demanding and more expensive to employed. A challenge for both platforms is the varied limit of detection (LoD) for target genomic loci, even within the same gene. The variability of detection sensitivity may be problematic if well-known actionable somat

SUBMITTER: Cheng YW 

PROVIDER: S-EPMC6656700 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature