Reconstitution reveals how myosin-VI self-organises to generate a dynamic mechanism of membrane sculpting.

ABSTRACT: One enigma in biology is the generation, sensing and maintenance of membrane curvature. Curvature-mediating proteins have been shown to induce specific membrane shapes by direct insertion and nanoscopic scaffolding, while the cytoskeletal motors exert forces indirectly through microtubule and actin networks. It remains unclear, whether the manifold direct motorprotein-lipid interactions themselves constitute another fundamental route to remodel the membrane shape. Here we show, combining super-resolution-fluorescence microscopy and membrane-reshaping nanoparticles, that curvature-dependent lipid interactions of myosin-VI on its own, remarkably remodel the membrane geometry into dynamic spatial patterns on the nano- to micrometer scale. We propose a quantitative theoretical model that expla

SUBMITTER: Rogez B 

PROVIDER: S-EPMC6656732 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature