A proteomic study of cysteine protease induced cell death in anthers of male sterile tobacco transgenic plants.

ABSTRACT: Manifestation of male sterility in plants is an important requirement for hybrid seed production. Tapetum cell layer of anther is a primary target for genetic manipulation for male sterility. In our previous report, the targeted expression of Arachis cysteine protease in tapetum led to premature degeneration of tapetal layer that resulted in complete male sterility in transgenic tobacco plants. To correlate cysteine protease mediated cell death of tapetum, transmission electron microscopy (TEM) and proteomic pattern of anthers of cysteine protease induced male sterile plant were compared with the untransformed control plant. TEM study revealed the abnormal growth of tapetal cells exhibiting excessive vacuolization that synchronized with irregular exine wall formation of the microspo

SUBMITTER: Shukla P 

PROVIDER: S-EPMC6656835 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature