Terbium ion as RNA tag for slide-free pathology with deep-ultraviolet excitation fluorescence.

ABSTRACT: Deep-ultraviolet excitation fluorescence microscopy has enabled molecular imaging having an optical sectioning capability with a wide-field configuration and its usefulness for slide-free pathology has been shown in recent years. Here, we report usefulness of terbium ions as RNA-specific labeling probes for slide-free pathology with deep-ultraviolet excitation fluorescence. On excitation in the wavelength range of 250-300 nm, terbium ions emitted fluorescence after entering cells. Bright fluorescence was observed at nucleoli and cytoplasm while fluorescence became weak after RNA decomposition by ribonuclease prior to staining. It was also found that the fluorescence intensity at nucleoplasm increased with temperature during staining and that this temperature-dependent behavior resembled te

SUBMITTER: Kumamoto Y 

PROVIDER: S-EPMC6656878 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature