Insertion/deletion-activated frame-shift fluorescence protein is a sensitive reporter for genomic DNA editing.

ABSTRACT:

Background

Reporter methods to quantitatively measure the efficiency and specificity of genome editing tools are important for the development of novel editing techniques and successful applications of available ones. However, the existing methods have major limitations in sensitivity, accuracy, and/or readiness for in vivo applications. Here, we aim to develop a straight-forward method by using nucleotide insertion/deletion resulted from genome editing. In this system, a target sequence with frame-shifting length is inserted after the start codon of a cerulean fluorescence protein (CFP) to inactivate its fluorescence. As such, only a new insertion/deletion event in the target sequence will reactivate the fluorescence. This reporter is therefore termed as "Insertion/deletion-activa

SUBMITTER: Kumar A 

PROVIDER: S-EPMC6657097 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature