Detection of Wolbachia in field-collected Aedes aegypti mosquitoes in metropolitan Manila, Philippines.

ABSTRACT:

Background

Recent reports reveal the presence of Wolbachia in Ae. aegypti. Our study presents additional support for Wolbachia infection in Ae. aegypti by screening field-collected adult mosquitoes using two Wolbachia-specific molecular makers.

Methods

A total of 672 Ae. aegypti adult mosquitoes were collected from May 2014 to January 2015 in Metropolitan Manila. Each individual sample was processed and screened for the presence of Wolbachia by selected markers, Wolbachia-specific 16S rDNA and its surface protein (wsp), under optimized PCR conditions and sequenced.

Results

Totals of 113 (16.8%) and 89 (13.2%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. The Ae. aegpyti wsp sample sequences

SUBMITTER: Carvajal TM 

PROVIDER: S-EPMC6657204 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature