Dual DNA Barcoding for the Molecular Identification of the Agents of Invasive Fungal Infections.

ABSTRACT: Invasive fungal infections, such as aspergillosis, candidiasis, and cryptococcosis, have significantly increased among immunocompromised people. To tackle these infections the first and most decisive step is the accurate identification of the causal pathogen. Routine identification of invasive fungal infections has progressed away from culture-dependent methods toward molecular techniques, including DNA barcoding, a highly efficient and widely used diagnostic technique. Fungal DNA barcoding previously relied on a single barcoding region, the internal transcribed spacer (ITS) region. However, this allowed only for 75% of all fungi to be correctly identified. As such, the translational elongation factor 1α (TEF1α) was recently introduced as the secondary barcode region to close

SUBMITTER: Hoang MTV 

PROVIDER: S-EPMC6657352 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature