Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.

ABSTRACT: Epitranscriptomic regulation controls information flow through the central dogma and provides unique opportunities for manipulating cells at the RNA level. However, both fundamental studies and potential translational applications are impeded by a lack of methods to target specific RNAs with effector proteins. Here, we present CRISPR-Cas-inspired RNA targeting system (CIRTS), a protein engineering strategy for constructing programmable RNA control elements. We show that CIRTS is a simple and generalizable approach to deliver a range of effector proteins, including nucleases, degradation machinery, translational activators, and base editors to target transcripts. We further demonstrate that CIRTS is not only smaller than naturally occurring CRISPR-Cas programmable RNA binding systems but ca

SUBMITTER: Rauch S 

PROVIDER: S-EPMC6657360 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature