Crohn's disease is facilitated by a disturbance of programmed death-1 ligand 2 on blood dendritic cells.

ABSTRACT:

Objective

Crohn's disease (CD) is characterised by inflammation, predominantly associated with ilea. To investigate the basis for this inflammation in patients with CD, we examined dendritic cells (DC) which are pivotal for maintenance of immunological tolerance in the gut.

Methods

Ileal biopsies and blood DCs from CD patients and controls were examined by microscopy and flow cytometry for PD-L1 and PD-L2 expression, as PD-L1 has been implicated in colitis but the contribution of PD-L2 is less clear. In vitro studies, of blood samples from CD patients, were used to demonstrate a functional role for PD-L2 in disease pathogenesis.

Results

Quantitative microscopy of CD11c⁺ DCs in inflamed and noninflamed ilea from CD patient showed > 75% loss of these c

SUBMITTER: Faleiro R 

PROVIDER: S-EPMC6657371 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature