Dataset Information

Effect of different anaesthetic techniques on gene expression profiles in patients who underwent hip arthroplasty.

ABSTRACT:

Objectives

To investigate the modulation of genes whose expression level is indicative of stress and toxicity following exposure to three anaesthesia techniques, general anaesthesia (GA), regional anaesthesia (RA), or integrated anaesthesia (IA).

Methods

Patients scheduled for hip arthroplasty receiving GA, RA and IA were enrolled at Rizzoli Orthopaedic Institute of Bologna, Italy and the expression of genes involved in toxicology were evaluated in peripheral blood mononuclear cells (PBMCs) collected before (T0), immediately after surgery (T1), and on the third day (T2) after surgery in association with biochemical parameters.

Results

All three anaesthesia methods proved safe and reliable in terms of pain relief and patient recovery. Gene ontology analysis revealed that GA and mainly IA were associated with deregulation of DNA repair system and stress-responsive genes, which was observed even after 3-days from anaesthesia. Conversely, RA was not associated with substantial changes in gene expression.

Conclusions

Based on the gene expression analysis, RA technique showed the smallest toxicological effect in hip arthroplasty.

Trial registration

ClinicalTrials.gov number NCT03585647.

SUBMITTER: Alleva R

PROVIDER: S-EPMC6657832 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Effect of different anaesthetic techniques on gene expression profiles in patients who underwent hip arthroplasty.

Alleva Renata R Tognù Andrea A Tomasetti Marco M Benassi Maria Serena MS Pazzaglia Laura L van Oven Hanna H Viganò Ettore E De Simone Nicola N Pacini Ilaria I Giannone Sandra S Gagic Sanjin S Borghi Raffaele R Picone Sara S Borghi Battista B

PloS one 20190725 7

<h4>Objectives</h4>To investigate the modulation of genes whose expression level is indicative of stress and toxicity following exposure to three anaesthesia techniques, general anaesthesia (GA), regional anaesthesia (RA), or integrated anaesthesia (IA).<h4>Methods</h4>Patients scheduled for hip arthroplasty receiving GA, RA and IA were enrolled at Rizzoli Orthopaedic Institute of Bologna, Italy and the expression of genes involved in toxicology were evaluated in peripheral blood mononuclear cel ...[more]

PMID: 31344051

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Project description:AimHow reduced femoral neck anteversion alters the distribution of pressure and contact area in Hip Resurfacing Arthroplasty (HRA) remains unclear. The purpose of this study was to quantitatively describe the biomechanical implication of different femoral neck version angles on HRA using a finite element analysis.Materials and methodsA total of sixty models were constructed to assess the effect of different femoral neck version angles on three different functional loads: 0°of hip flexion, 45°of hip flexion, and 90° of hip flexion. Femoral version was varied between 30° of anteversion to 30° of retroversion. All models were tested with the acetabular cup in four different positions: (1) 40°/15° (inclination/version), (2) 40°/25°, (3) 50°/15°, and (4) 50°/25°. Differences in range of motion due to presence of impingement, joint contact pressure, and joint contact area with different femoral versions and acetabular cup positions were calculated.ResultsImpingement was found to be most significant with the femur in 30° of retroversion, regardless of acetabular cup position. Anterior hip impingement occurred earlier during hip flexion as the femur was progressively retroverted. Impingement was reduced in all models by increasing acetabular cup inclination and anteversion, yet this consequentially led to higher contact pressures. At 90° of hip flexion, contact pressures and contact areas were inversely related and showed most notable change with 30° of femoral retroversion. In this model, the contact area migrated towards the anterior implant-bone interface along the femoral neck.ConclusionFemoral retroversion in HRA influences impingement and increases joint contact pressure most when the hip is loaded in flexion. Increasing acetabular inclination decreases the area of impingement but doing so causes a reciprocal increase in joint contact pressure. It may be advisable to study femoral neck version pre-operatively to better choose hip resurfacing arthroplasty candidates.

Dataset Information

Effect of different anaesthetic techniques on gene expression profiles in patients who underwent hip arthroplasty.

Objectives

Methods

Results

Conclusions

Trial registration

Publications

Effect of different anaesthetic techniques on gene expression profiles in patients who underwent hip arthroplasty.

Similar Datasets

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