Abcc5 Knockout Mice Have Lower Fat Mass and Increased Levels of Circulating GLP-1.

ABSTRACT:

Objective

A previous genome-wide association study linked overexpression of an ATP-binding cassette transporter, ABCC5, in humans with a susceptibility to developing type 2 diabetes with age. Specifically, ABCC5 gene overexpression was shown to be strongly associated with increased visceral fat mass and reduced peripheral insulin sensitivity. Currently, the role of ABCC5 in diabetes and obesity is unknown. This study reports the metabolic phenotyping of a global Abcc5 knockout mouse.

Methods

A global Abcc5^-/- mouse was generated by CRISPR/Cas9. Fat mass was determined by weekly EchoMRI and fat pads were dissected and weighed at week 18. Glucose homeostasis was ascertained by an oral glucose tolerance test, intraperitoneal glucose tolerance test, and intraperitonea

SUBMITTER: Cyranka M 

PROVIDER: S-EPMC6658130 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature