A complex human gut microbiome cultured in an anaerobic intestine-on-a-chip.

ABSTRACT: The diverse bacterial populations that comprise the commensal microbiome of the human intestine play a central role in health and disease. A method that sustains complex microbial communities in direct contact with living human intestinal cells and their overlying mucus layer in vitro would thus enable the investigation of host-microbiome interactions. Here, we show the extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients. When compared to aerobic coculture conditions, the establishment of a transluminal hypoxia gradient in the chip increased intestinal barrier function and sustaine

SUBMITTER: Jalili-Firoozinezhad S 

PROVIDER: S-EPMC6658209 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature