3D-structured supports create complete data sets for electron crystallography.

ABSTRACT: 3D electron crystallography has recently attracted much attention due to its complementarity to X-ray crystallography in determining the structure of compounds from submicrometre sized crystals. A big obstacle lies in obtaining complete data, required for accurate structure determination. Many crystals have a preferred orientation on conventional, flat sample supports. This systematically shades some part of the sample and prevents the collection of complete data, even when several data sets are combined. We introduce two types of three-dimensional sample supports that enable the collection of complete data sets. In the first approach the carbon layer forms coils on the sample support. The second approach is based on chaotic nylon fibres. Both types of grids disrupt the preferred orientati

SUBMITTER: Wennmacher JTC 

PROVIDER: S-EPMC6658500 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature