Pharmacological Profiling of Purified Human Stem Cell-Derived and Primary Mouse Motor Neurons.

ABSTRACT: Directed differentiation of human pluripotent stem cells (hPSCs) has enabled the generation of specific neuronal subtypes that approximate the intended primary mammalian cells on both the RNA and protein levels. These cells offer unique opportunities, including insights into mechanistic understanding of the early driving events in neurodegenerative disease, replacement of degenerating cell populations, and compound identification and evaluation in the context of precision medicine. However, whether the derived neurons indeed recapitulate the physiological features of the desired bona fide neuronal subgroups remains an unanswered question and one important for validating stem cell models as accurate functional representations of the primary cell types. Here, we purified both hPSC-derived an

SUBMITTER: Moakley D 

PROVIDER: S-EPMC6658502 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature