Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum.

ABSTRACT: Like many viruses, rotavirus (RV) dysregulates calcium homeostasis by elevating cytosolic calcium ([Ca²⁺]cyt) and decreasing endoplasmic reticulum (ER) stores. While an overall, monophasic increase in [Ca²⁺]cyt during RV infection has been shown, the nature of the RV-induced aberrant calcium signals and how they manifest over time at the single-cell level have not been characterized. Thus, we generated cell lines and human intestinal enteroids (HIEs) stably expressing cytosolic and/or ER-targeted genetically-encoded calcium indicators to characterize calcium signaling throughout RV infection by time-lapse imaging. We found that RV induces highly dynamic [Ca²⁺]cyt signaling that manifest as hundreds of discrete [Ca²⁺]cyt spikes, which increase dur

SUBMITTER: Chang-Graham AL 

PROVIDER: S-EPMC6658527 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature