Orai1 inhibitor STIM2β regulates myogenesis by controlling SOCE dependent transcriptional factors.

ABSTRACT: Store-operated Ca²⁺ entry (SOCE), the fundamental Ca²⁺ signaling mechanism in myogenesis, is mediated by stromal interaction molecule (STIM), which senses the depletion of endoplasmic reticulum Ca²⁺ stores and induces Ca²⁺ influx by activating Orai channels in the plasma membrane. Recently, STIM2β, an eight-residue-inserted splice variant of STIM2, was found to act as an inhibitor of SOCE. Although a previous study demonstrated an increase in STIM2β splicing during in vitro differentiation of skeletal muscle, the underlying mechanism and detailed function of STIM2β in myogenesis remain unclear. In this study, we investigated the function of STIM2β in myogenesis using the C2C12 cell line with RNA interference-mediated knockdown and CRISPR-Cas-medi

SUBMITTER: Kim KM 

PROVIDER: S-EPMC6658661 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature