The molecular mechanism of LncRNA34a-mediated regulation of bone metastasis in hepatocellular carcinoma.

ABSTRACT:

Background

Bone metastasis (BM) has long been recognized as a major threat to the quality of life of hepatocellular cancer (HCC) patients. While LncRNA34a (Lnc34a) has been shown to regulate colon cancer stem cell asymmetric division, its effect on HCC BM remains unknown.

Methods

In situ hybridization and quantitative real-time polymerase chain reaction (qRT-PCR) were used to detect the expression of Lnc34a in HCC tissues and cell lines. Ventricle injection model was constructed to explore the effect of Lnc34a on BM in vivo. The methylation of miR-34a promoter and histones deacetylation were examined by using bisulfate-sequencing PCR and chromatin immunoprecipitation assays. RNA pull down and RNA immunoprecipitation were performed to investigated the interaction between Lnc3

SUBMITTER: Zhang L 

PROVIDER: S-EPMC6659280 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature