SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways.

ABSTRACT:

Background

The SUMO-activating enzyme SAE1 is indispensable for protein SUMOylation. A dysregulation of SAE1 expression involves in progression of several human cancers. However, its biological roles of SAE1 in glioma are unclear by now.

Methods

The differential proteome between human glioma tissues and para-cancerous brain tissues were identified by LC-MS/MS. SAE1 expression was further assessed by immunohistochemistry. The patient overall survival versus SAE1 expression level was evaluated by Kaplan-Meier method. The glioma cell growth and migration were evaluated under SAE1 overexpression or inhibition by the CCK8, transwell assay and wound healing analysis. The SUMO1 modified target proteins were enriched from total cellular or tissue proteins by incubation with the anti

SUBMITTER: Yang Y 

PROVIDER: S-EPMC6659289 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature