Proof of concept for quantitative urine NMR metabolomics pipeline for large-scale epidemiology and genetics.

ABSTRACT:

Background

Quantitative molecular data from urine are rare in epidemiology and genetics. NMR spectroscopy could provide these data in high throughput, and it has already been applied in epidemiological settings to analyse urine samples. However, quantitative protocols for large-scale applications are not available.

Methods

We describe in detail how to prepare urine samples and perform NMR experiments to obtain quantitative metabolic information. Semi-automated quantitative line shape fitting analyses were set up for 43 metabolites and applied to data from various analytical test samples and from 1004 individuals from a population-based epidemiological cohort. Novel analyses on how urine metabolites associate with quantitative serum NMR metabolomics data (61 metabolic measure

SUBMITTER: Tynkkynen T 

PROVIDER: S-EPMC6659374 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature