An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.

ABSTRACT: The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and

SUBMITTER: Narimatsu Y 

PROVIDER: S-EPMC6660356 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature