Stochastic geometry sensing and polarization in a lipid kinase-phosphatase competitive reaction.

ABSTRACT: Phosphorylation reactions, driven by competing kinases and phosphatases, are central elements of cellular signal transduction. We reconstituted a native eukaryotic lipid kinase-phosphatase reaction that drives the interconversion of phosphatidylinositol-4-phosphate [PI(4)P] and phosphatidylinositol-4,5-phosphate [PI(4,5)P₂] on membrane surfaces. This system exhibited bistability and formed spatial composition patterns on supported membranes. In smaller confined regions of membrane, rapid diffusion ensures the system remains spatially homogeneous, but the final outcome-a predominantly PI(4)P or PI(4,5)P₂ membrane composition-was governed by the size of the reaction environment. In larger confined regions, interplay between the reactions, diffusion, and confinement crea

SUBMITTER: Hansen SD 

PROVIDER: S-EPMC6660746 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature