Design of a multi-sensor platform for integrating extracellular acidification rate with multi-metabolite flux measurement for small biological samples.

ABSTRACT: Rates of cellular oxygen consumption (OCR) and extracellular acidification (ECAR) are widely used proxies for mitochondrial oxidative phosphorylation (OXPHOS) and glycolytic rate in cell metabolism studies. However, ECAR can result from both oxidative metabolism (carbonic acid formation) and glycolysis (lactate release), potentially leading to erroneous conclusions about metabolic substrate utilization. Co-measurement of extracellular glucose and lactate flux along with OCR and ECAR can improve the accuracy and provide better insight into cellular metabolic processes but is currently not feasible with any commercially available instrumentation. Herein, we present a miniaturized multi-sensor platform capable of real-time monitoring of OCR and ECAR along with extracellular lactate and glucos

SUBMITTER: Obeidat YM 

PROVIDER: S-EPMC6660976 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature