SHG/TPEF-based image technology improves liver fibrosis assessment of minimally sized needle biopsies.

ABSTRACT:

Background and aims

Sampling size variability of liver biopsy remains a major limitation in the assessment of liver fibrosis. We aimed to evaluate the diagnostic value of a fully quantitative method (second harmonic generation/two-photon excitation fluorescence, SHG/TPEF based) in "short" liver biopsy samples.

Methods

Liver biopsy samples from chronic hepatitis B (CHB) patients were constructed into "virtual" biopsies with different lengths. The original and "virtual" samples were measured by SHG/TPEF-based technology to obtain qFibrosis score, respectively. Here, ΔqFibrosis was defined as difference of qFibrosis between original biopsy and "virtual" biopsy. Equivalence test was used to compare ΔqFibrosis with the clinically acceptable error (deviation of 0.50) in each group

SUBMITTER: Wang B 

PROVIDER: S-EPMC6661026 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature